Adapted MS/MSALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species


January 1, 2018

Lipids. 2018 Jan;53(1):133-142

Fei Gao, Justice McDaniel, Emily Y. Chen, Hannah E. Rockwell, Cindy Nguyen, Vivek Vishnudas, Matthew D. Lynes, Yu-Hua Tseng, Rangaprasad Sarangarajan, Niven R. Narain, Michael A. Kiebish

Cardiolipin (Ptd2Gro) is a complex, double-charged phospholipid located in the inner mitochondrial membrane where it plays an essential role in regulating bioenergetics.  Abnormalities in CL content or composition have been associated with mitochondrial dysfunction in a variety of disease states.  Herein, we developed an adapted high-resolution data independent acquisition (DIA) MS/MSALL shotgun lipidomic method to enhance the accuracy and reproducibility of Ptd2Gro molecular species quantitation from biological samples. Utilizing the double charged molecular ions and the isotopic pattern with negative electrospray mode ionization mass spectrometry (ESI-MS) using an adapted MS/MSALL approach, we profiled more than 150 individual CL species, including monolysocardiolipin (MLCL). The method described in this study demonstrated high reproducibility, sensitivity, and throughput with a wide dynamic range. This high-resolution MS/MSALL shotgun lipidomics approach could be extended to screening aberrations of CL metabolism involved in mitochondrial dysfunction in various pathological conditions and diseases.

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